03/23 PCRs
Ladder; CD79A; CD79B; pTet:mRFP | Notes: CD79A and CD79B bands are not of the correct size. They appear to have primer dimers. Temperature used for the PCR may not be appropriate, since we changed PCR reagents from Phusion to Q5. Gal4VP16 and pTet:mRFP construct might be correct - too much DNA in the lane to discern exact position of the band. PCRs will be redone with correct temperatures and less template. |
03/31 and 04/01 PCRS
 |  | Notes:(Lanes 1-5 had holes in the bottom, had to load additional lanes because first samples were lost - no additional DNA from PCR reaction) Gal4VP16 and pTet:mRFP bands are the correct size CD79A and CD79B constructs show bands of smaller size. Since we're redoing the Gal4VP16 and pTet:mRFP PCRs anyway we can redo the CD79 PCRs but increase the annealing temperature by 2C |
..but we have to redo the PCR reaction because we don't have any PCR product left. 04/10 PCRs
|  | Notes:Gal4VP16 and pTet:mRFP constructs have the correct band size --> ready for AarI golden gate CD79A and CD79B PCRs still seem to have primer dimers.CD79A/B primers have to be redesigned. |