hEF1a:eYFP | TRE:eYFP | TRE:mKate | hEF1a:CD79A-RFP-G4 SDM #1 | hEF1a:CD79A-RFP-G4 SDM #2 | hEF1a:CymR | hEF1a-CuO:mKate | CMV5-CuO:mKate | hEF1a:CD79A-3T3-G4 1,2,3,4 | hEF1a:CD79A-3T9-G4 1,2 | hEF1a:CD79A-3T15-G4 1,2,3,4 | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | , 10, 11, 12 | 13, 14 | 15, 16, 17, 1811 |
| Enzyme | ApaI, NcoI | BlpI, XbaI | StuI | BsaI | BsaI | PvuII | PvuII | PvuII | |||||
| Virtual gel |  |  |  | Fail
Success bands 5 kb 3.8 kb 0.85 kb | Fail
Success bands 5 kb 3.8 kb 0.85 kb |  | | | |||||
| Concentration (ng/uL) | |||||||||||||
| Buffer | |||||||||||||
| Enzyme | |||||||||||||
| DNA volume | |||||||||||||
| H2O volume | |||||||||||||
| Buffer volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | ||
| Enzyme volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
...