hEF1a:CD79AeYFPhEF1a | TRE:CD79BeYFP | hEF1a:Lyn | hEF1a:GMabHTRE:mKate | hEF1a:GMabLhEF1a:CD79A-TCSRFP-G4 SDM #1 | hEF1a:CD79BCD79A-TCSRFP-G4 | TRE:Syk-15-TEVp | SDM #2 | hEF1a:CymRhEF1a:Gal4VP16 | hEF1a-LacOCuO:mKate | TRECMV5-CuO:mKate | hEF1a: | LacICD79A-3T3-G4 #1 | hEF1a:CD79A-3T9-G4 #1 | hEF1a:CD79A-3T15-G4 #1 | TRE:Gal4VP16 | pENTR hEF1a-LacO | pENTR LacI | TRE:Syk||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | ||||||||||||||||||||||||||||
| Enzyme | ApaI Cuts in hEF1a, CD79A & backbone | ApaI Cuts in hEF1a, and backbone | ApaI, NcoI Cuts in Lyn, hEF1a and backbone | BglII Cuts in GmabH, hEF1a and backbone | KpnI Cuts in GmabL, hEF1a, and backbone | PvuII Cuts in CD79A, TCS and backbone | PvuII Cuts in CD79B, TCS and backbone | PvuII Cuts in syk, tevp and backbone | ApaI Cuts in hEF1a, rtTA3 and backbone | XhoI Cuts in hEF1a, Gal4VP16 and backbone | NheI | PvuII | ApaI | XhoI | ApaI cuts in hEF1a-LacO | ApaI cuts in LacI | PvuII cuts in syk | Virtual gel |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | | |||||||||||
| Virtual gel | |||||||||||||||||||||||||||||||||||||||||||||
| Concentration (ng/uL) | 1110.3 | 1420 | 1314.4 | 983.5 | 1359.0 | 1086.1 | 487.2 | 1161.9 | 706.4 | 441.9 | 741.0 | 483.1 | 420.6 | 1265.3 | 229.8 | 196.7 | 321.8 | Buffer||||||||||||||||||||||||||||
| Buffer | Enzyme | ||||||||||||||||||||||||||||||||||||||||||||
| Enzyme | |||||||||||||||||||||||||||||||||||||||||||||
| DNA volume | 0.45032874 | 0.352112676 | 0.3804017 | 0.508388409 | 0.367917586 | 0.460362766 | 1.026272578 | 0.430329632 | 0.70781427 | 1.13147771 | 0.674763833 | 1.03498241 | 1.18877794 | 0.395163202 | 2.175805048 | 2.54194204 | 1.55376 | ||||||||||||||||||||||||||||
| H2O volume | 11.54967126 | 11.64788732 | 11.619598 | 11.49161159 | 11.63208241 | 11.53963723 | 10.97372742 | 11.56967037 | 11.2921857 | 10.86852229 | 11.32523617 | 10.9650176 | 10.8112221 | 11.6048368 | 9.824194952 | 9.45805796 | 10.44624 | ||||||||||||||||||||||||||||
| H2O volume | |||||||||||||||||||||||||||||||||||||||||||||
| Buffer volume | Buffer volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.51.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | ||||||||||||||||||||||||||||
| Enzyme volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.51.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
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