...
| hEF1a:CD79A-linker-Gal4VP16 | 3T3 | 3T9 | 3T15 | 6T6 | 6T9 | 6T12 | 9T3 | 9T6 | 9T9 | 9T12 | 9T15 | 12T6 | 12T9 | 12T12 | 15T3 | 15T9 | 15T15 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Letter | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q |
| Concentration (ng/uL) | 18 | 38.3 | 35.3 | 25.1 | 31.0 | 26.8 | 209.9 | 402.9 | 175.6 | 249.1 | 311.6 | 29.2 | 27.2 | 32.7 | 17.6 | 19.3 | 23.5 |
| DNA volume | 12 | 12 | 12 | 12 | 12 | 12 | 2.382087 | 1.241003 | 2.84738 | 2.007226 | 1.604621 | 12 | 12 | 12 | 12 | 12 | 12 |
| H2O volume | 0 | 0 | 0 | 0 | 0 | 0 | 9.617913 | 10.759 | 9.15262 | 9.992774 | 10.39538 | 0 | 0 | 0 | 0 | 0 | 0 |
| Buffer volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Enzyme volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
...
hEF1a:CD79A-TCS-G4
...
hEF1a:CD79B-TCS-G4
...
TRE:Syk-15-TEVp
...
...
hEF1a:Gal4VP16
...
...
...
UAS:eYFP
...
UAS:mKate
...
...
TRE:mKate
...
hEF1a:LacI
...
TRE:Gal4VP16
| 1-10 | 11-17 | A-J | K-Q |
 |  |  |  |
...
pENTR hEF1a-LacO
...