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changes.mady.by.user Unknown User (eersland@mit.edu)

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pEXPR 1-pLac:mKate-2

pEXPR TRE:Syk-pTet:RFP-TEVTEVp

pEXPR TRE:Syk-pTet:RFP-tTEVp

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all three tTEV constructs are correct. TEV #3 is correct

tube name	Constants Definition	2-log ladder	pEXPR 1-pLac:mKate-2	pEXPR 1-pLac:mKate-2	pEXPR 1-pLac:mKate-2	pEXPR 1-pLac:mKate-2	pEXPR 1-pLac:mKate-2	pEXPR 1-pLac:mKate-2	pEXPR 1-pLac:mKate-2	pEXPR TRE:Syk-pTet:RFP-tTEV	pEXPR TRE:Syk-pTet:RFP-tTEV	pEXPR TRE:Syk-pTet:RFP-tTEV	pEXPR TRE:Syk-pTet:RFP-TEV	2-log ladder	pEXPR TRE:Syk-pTet:RFP-TEV	pEXPR TRE:Syk-pTet:RFP-TEV	pEXPR TRE:Syk-pTet:RFP-TEV
Tube Number			1/1	1/2	1/3	2/1	2/2	2/3	1/2	B1	B2	B3	C1		C2	C3	C2
gel lane	0	1/1	1/2	1/3	1/4	1/5	1/6	1/7	1/8	1/9	1/10	1/11	1/12	NaN	2/1	2/2	2/3
DNA mass desired (ng)	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500
total volume(ul)	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20
DNA concentration (ng/ul)			332.8	253.5	358.6			360.9	253.5	245.9	294.3	280.5	338.5		213.9	254.8	213.9
Enzyme Name			NheI	NheI	NheI	NheI	NheI	NheI	undigested	PvuII	PvuII	PvuII	PvuII		PvuII	PvuII	undigested
Buffer Name			2.1	2.1	2.1	2.1	2.1	2.1	water	3.1	3.1	3.1	3.1		3.1	3.1	water
gel/lane	0	1/1	1/2	1/3	1/4	1/5	1/6	1/7	1/8	1/9	1/10	1/11	1/12	NaN	2/1	2/2	2/3
DNA volume		#DIV/0!	1.50	1.97	1.39	#DIV/0!	#DIV/0!	1.39	1.97	2.03	1.70	1.78	1.48	#DIV/0!	2.34	1.96	2.34
buffer volume	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2
enzyme volume	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
strip tube #		1/1	1/2	1/3	1/4	1/5	1/6	1/7	1/8	1/9	1/10	1/11	1/12	2/1	2/2	2/3	2/4
water volume	17.0	#DIV/0!	15.5	15.0	15.6	#DIV/0!	#DIV/0!	15.6	15.0	15.0	15.3	15.2	15.5	#DIV/0!	14.7	15.0	14.7
gel/lane	0	1/1	1/2	1/3	1/4	1/5	1/6	1/7	1/8	1/9	1/10	1/11	1/12	NaN	2/1	2/2	2/3
loading dye	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4
results			good	bad, pZDonor	good	bad pick	bad pick	bad		good	good	good	???		contaminated	good
notes

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