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SDM3 - Correcting CD79B Point Mutation from PCR (Same as above, different protocol) - Golden Gate Mutagenesis Q5 protocol 5 min elongation time, 67C annealing temperature

 

 

LR

hEF1a:CymR

hEF1a-CuO:mKate

CMV5-Cuo:mKate

(pDEST_Seq2-Seq3)