...
- Antibody amino acid (or DNA) sequence is available.
- Antigen (for activating system) is commercially available at a reasonable cost.
- Antigens should not significantly affect our mammalian cells other than their involvement in activating our system (for example, avoid EGFR or TNF-alpha as antigens). Additionally, antigens shouldn't present significant health dangers to us (for example, anthrax spores).
- Antigen-antibody interaction should be well-characterized.
Antibody databases: (don't know when these pages were last updated)
IBt (list of antibodies in PDB): http://www.ibt.unam.mx/vir/structure/3directory.html
IMGT (list of sequenced antibodies): http://www.imgt.org/mAb-DB/query
Summary Table
| Antigen | MAb name | Peptide available? | gBlocks ready? |
|---|---|---|---|
| Hen egg lysozyme | HyHEL-10 | Yes | Yes |
| HIV gp120 | 0.5beta | Kind of | Didn't optimize with IDT for gBlocks, should check IgK sequence |
| HIV gp120 | VRC01 | 99.9% sure | Yes |
...