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| Ladder, TRE:mKate, pLac:mKate, hEF1a:LacI, 3T3, 3T9, 3T15, 6T6, 6T9, 6T12 | Ladder, 9T3, 9T6, 9T9, 9T12, 9T12, 9T15, 12T6, 12T9, 12T12, 15T3, 15T9, 15T15 | Notes: |
 |  | The LRed constructs (1st 3 lanes after the ladder, left gel) seem to be in the wrong order. The band pattern in lane 1may be the failure mode of TRE:mKate, but there is an extra band at around 10kb which doesn't make sense, also there seems to be some undigested plasmid as well. The band pattern in lane 2 is probably the correct hEF1a:LacI, even though it says it's pLac:mKate. The band pattern in lane 3 is probably the correct pLac:mKate, even though it says it's hEF1a:LacI In gel 2, all of the linker constructs have the correct band pattern, except that some of them have an additional band at around 4-5 kb. We sent 9TCS9 for sequencing, if it is correct we'll send the rest of them for sequencing. In gel 1, 3TCS3 and 3TCS9 have the correct band pattern. The rest also have the extra 4-5 kb band and the an expected 1.5 band is not visible. |
Sent For Sequencing:
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