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enzymes:

pEXPR 1-Hef1a:LacI-2 pEXPR 1-TRE mKate-2pEXPR 1-pLac mKate-2 hef1a:cd79A-3T3-gal4
ApaI SalI ApaIBglII, MluIXbaI
 Image Added Image Added Image Added