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Immediate To Do's:
  •  look over syk linker in silico cloning 
  •  update timeline
  •  miniprep CD79A linkers miniprep
  •  TRE:mKate, pLac:mKate, hEF1a:LacI miniprep
  •  digests and gels 
  •  TRE:Gal4VP16 LR

BCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

 

 

Higher Level To Do's:
  •  Decide on other antigen-variable regions we want to test  
  •  Other experimental planning (on experimental page)
  •  How do we do site directed mutagenesis?
  •  Literature research: 
    •  kozak sequences for heavy and light chains and CD79s
    •  truncated TEVp
    •  other antigens to detect using BCR (we were thinking of small molecule detection)
    •  inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first) 
  •  Cloning planning 
    •  hEF1a:Syk-pTet:mRFP-TEVp
    •  variable region