BCR 2.0
Experimental Progress:
Cloning Progress
Parts Tree
Cloning Strategy
Experiments
Linker Optimization
Antibody Choices
Useful Links:
Protocols
Immediate To Do's:
- kill tret:vp16gal4 LR
- transform tret:vp16gal4 LR
- pick colonies from CD79A linker GG plates
- pick colonies from LR transforms (pLac:mKate, h:LacI, tret:mKate
- look over syk linker in silico cloning
- update timeline
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
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| title | Variable region construction |
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Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Other experimental planning (on experimental page)
- How do we do site directed mutagenesis?
- Literature research:
- kozak sequences for heavy and light chains and CD79s
- truncated TEVp
- other antigens to detect using BCR (we were thinking of small molecule detection)
- inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first)
- Cloning planning
- hEF1a:Syk-pTet:mRFP-TEVp
- variable region