Melting Temperature

Link to Tm Calculator: https://www.neb.com/tools-and-resources/interactive-tools/tm-calculator

Product Group: Phusion

Product: Phusion High-Fidelity PCR Master Mix (HF Buffer)

Primer Conc. (nM): 500

For the primer sequences, input only the binding region and not the entire primer.

For PCR protocol: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531

Hairpins and dimers

IDT's Oligoanalyzer:  http://www.idtdna.com/calc/analyzer

How to interpret the delta G values you get:  http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html