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- Supplementary materials includes VRC01 variable regions' amino acid sequences
- Supplementary materials includes HXB2 strain gp120 core reference sequences that were used for screening antibodies (Fig. S1B). They consisted of the core sequences separated by GAG linkers.
There is a mutation from serine at position 334 in commercial peptides (from env HXB2 sequences) to alanine in this paper. However, many , but:- Many of the gp120 core mutants they
- designed and analyzed computationally had a threonine at this position (334), including the one they
- eventually chose for in vitro screening (RSC3).
- Based on this information, it seems that the amino acid present at this position isn't critical to binding, and that binding can still happen even when
- alanine is replaced by a hydroxyl
- -containing amino acid like threonine (so by extension maybe serine is ok too).
- The reference sequence for YU2 strain gp120 also has a serine at the position corresponding to 334 in HXB2 (contained within a conserved amino acid sequence), and VRC01 is shown to have bound YU2 gp120 (wild-type) well (Table S1).
- The position in JRCSF strain gp120 that corresponds to 334 in HXB2 is not identified as a binding site in the binding information paper (see binding information paper, Fig 1 - in fact it's not even included in the table).
- Has numbers about binding interactions (including Kd!)
- SPR
- ITC
- Competition ELISA
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