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- Supplementary materials includes VRC01 variable regions amino acid sequences
- Supplementary materials includes HXB2 strain gp120 core reference sequences that were used for screening antibodies (Fig. S1B). Only includes core parts They consisted of the gp120 amino acid sequence core sequences separated by GAG linkers.
One mutation S334A relative to There is a mutation from serine at position 334 in commercial peptides (from env HXB2 sequences of commercially available peptdies. ) to alanine in this paper. However, many of the gp120 core mutants they used in their computation screen had a threonine at this position, including the one they primarily used in screening (RSC3). It seems that the amino acid present at this position isn't critical to binding, and that binding can still happen even when nonpolar alanine is replaced by a hydroxyl group containing amino acid. - Has numbers about binding interactions (including Kd!)
- SPR
- ITC
- Competition ELISA
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