Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Chicken lysozyme derives from chicken egg white.  Breaks down bacterial cell walls.

mAb name:  HyHEL-10

Peptide suppliers:

Sigma-Aldrich:  1g/$49.50

...

**PROBLEM:  These have the amino acid sequence RKRIRIQRGPGRAFVTIG

 

Tried testing binding in silico:

Performed in silico mutation using Swiss PDB Viewer:  S234R, where 234 is PDB residue number (contained in peptide P) corresponding to S residue in RKSIRIQRGPGRAFVTIG

Energy minimization /repacking with Python code from 20.320 using PyRosetta.

But, looking more closely at PDB file SPV didn't add hydrogens with the mutation (sad)