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BCR 2.0
Things to do:
| Task | Claimed by | Date Completed | Other Notes |
|---|---|---|---|
| CD79A/B, Gal4VP16, RFP PCR try3 | CR | Apr 10 |
|
| Gel verification of try3 PCR product | LA | Apr 10 | RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers |
| Redesign primers | LA + CR | Apr 11 | CD79A F and R and CD79B F primers were redesigned and ordered |
| CD79s PCR with new primers | |||
| Gel verification of CD79 PCRs with new primers | |||
| AarI golden gates |
To Do:
- Redesign CD79B PCR primers
- AarI Golden GateCD79A-pTet:RFP-Gal4VP16
- Gel Extraction of Q1-CD79A-Q3
- Column Purification of pTet-RFP and Gal4VP16 PCR products
- Digest & Gel of CD79A-pTet: RFP - Gal4VP16
- Establish Cryostocks of GmabH, GmabL, Syk-Tev, and Lyn-Tev pEXPRs and dilute DNA to 200 ng/uL
Useful Links
| Protocols |
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