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  • C. japonicus is able to utilize corn stover and switchgrass as sole sources of carbon and energy for growth. 
  • efficient cellulase secretion and growth on biomass are prevented by disruption of the type II secretion system

FIG. 2.Image Added

GENETIC MANIPULATION

  • C. japonicus can be metabolically engineered using broad-host-range plasmids.
  • We developed tools for directed gene disruptions in C. japonicus and used this system to construct a mutant in the gspD gene, which is predicted to encode a component of the type II secretion system. The gspD::pJGG1 mutant displayed a greater-than-2-fold decrease in endoglucanase secretion compared to wild- type C. japonicus. In addition, the mutant strain showed a pronounced growth defect in medium with biomass as a carbon source, yielding 100-fold fewer viable cells than the wild type. 

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