| Construct | F Tm | R Tm | Rxn T |
|---|---|---|---|
| CD79A | 69.2 | 70.7 | 70 |
| CD79B | 69.1 | 73 | 71 |
Construct | Expected Band Size |
|---|---|
| CD79A | 750 bp |
| CD79B | 750 bp |
Notes:
CD79A, pTet:RFP, Gal4VP16 constructs have the correct band size --> ready for AarI golden gate
CD79B PCRs still seem to have primer dimers. CD79B primers have to be redesigned.