...
- Group 1: cellulose -> intermediate via ?
- growth conditions
- measure byproducts and intermediate metabolites
- growth dynamics
- effects of pH, temperature, feed stock, inhibitory byproducts
- Ubq ligase + Ab -> knockdown
- genetic manipulations (e.g. can we express GFP?)
- Group 2: intermediate -> biodiesel via E. coli
- effects of pH, temperature, intermediates from bacteria #1, inhibitory byproducts
- measure biodiesel (make an assay?)
- Biodiesel transporter: measure internal and external FAEE
- Which genes turn intermediates into biodiesel? construction of plasmids? regulation of genes?
Need to know for next week:
- Which intermediates? Which pathways of cellulose production?