...
- Is well-studied and well-characterized; lots of past work in E. coli
- Past iGEM teams
- Nevada 2011: "A Cooperative Relationship Between Cyanobacteria and E.Coli for Production of Biofuels"
- separate populations of cyanobacteria and E. Coli
- http://2011.igem.org/Team:Nevada
- WISE 2011
- Nevada 2011: "A Cooperative Relationship Between Cyanobacteria and E.Coli for Production of Biofuels"
- difference between diesel and gasoline
- diesel freezes at a lower temperature than gasoline (used in Canadian cars)
- diesel doesn't need a spark; just need compression
- making gasoline is more complicated; more complicated mixture
- diesel is more efficient, but less powerful
- What is biodiesel:
- main component is sulfur ester
- fatty acid methyl esters (FAME)
- fatty acid ethyl esters (FAEE)
- pathway:
- glycolysis -> pyruvate -> acetyl coA -> Fatty acid synthesis -> fatty acid esters
- glycolysis -> pyruvate -> acetaldehyde -> ethanol
- How to get biodiesel out of cell?
- need to lyse cells?
- engineer bacteria to excrete it and then skim it off the top of the reactor? -E. coli exporters
- Bioenergy landscape
- Stephanopoulos Lab - http://bamel.scripts.mit.edu.ezproxyberklee.flo.org/gns/research/biofuels/
- UROP posting:
- Project Title: Synthetic Biology Approaches to the Isoprenoid Pathway for the Production of Drugs and Biofuels
- Contact: Steven Edgar (sedgar@mit.edu)
- UROP posting:
- Stephanopoulos Lab - http://bamel.scripts.mit.edu.ezproxyberklee.flo.org/gns/research/biofuels/
- Which genes? Where do we get them?
- Are they in the iGEM kit?
- How to measure?
- "The fatty acid and ethanol assays were quantitated by generating standard curves of absorbance vs substrate concentration using known quantities of palmitic acid or ethanol standards. "
...
- Group 1: cellulose -> intermediate via ?
- growth conditions
- measure byproducts and intermediate metabolites
- growth dynamics
- effects of pH, temperature, feed stock, inhibitory byproducts
- Ubq ligase + Ab -> knockdown
- genetic manipulations (e.g. can we express GFP?)
- Group 2: intermediate -> biodiesel via E. coli
- effects of pH, temperature, intermediates from bacteria #1, inhibitory byproducts
- measure biodiesel (make an assay?)
- Biodiesel transporter: measure internal and external FAEE
- Which genes turn intermediates into biodiesel? construction of plasmids? regulation of genes?
Need to know for next week:
- Which intermediates? Which pathways of cellulose production?