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  • Is well-studied and well-characterized
  • Past iGEM teams
    • Nevada 2011: "A Cooperative Relationship Between Cyanobacteria and E.Coli for Production of Biofuels"
    • WISE 2011
  • difference between diesel and gasoline
    • diesel freezes at a lower temperature than gasoline (used in Canadian cars)
    • diesel doesn't need a spark; just need compression
    • making gasoline is more complicated; more complicated mixture
    • diesel is more efficient, but less powerful 
  • What is biodiesel:
    • main component is sulfur ester
    • fatty acid methyl esters (FAME)
    • fatty acid ethyl esters (FAEE)
    • pathway:
      • glycolysis -> pyruvate -> acetyl coA -> Fatty acid synthesis -> fatty acid esters
      • glycolysis -> pyruvate -> acetaldehyde -> ethanol
      • Image Added
  • How to get biodiesel out of cell:
    • need to lyse cells?