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1. What does the interaction time between a T Cell and an APC look like for T Cells that bind the antigen the cell is presenting as opposed to those that do not bind?
http://www.bloodjournal.org/content/104/9/2801?sso-checked=true varies considerably in duration and kinetics for t-cell and APCs...
, we found that B cells required contact to T cells for up to 60 hours to fully activate naive T cells (Figure 3A). In contrast, T cells became independent of further antigen presentation after 2 to 6 hours of contact to DC (dendritic cells)
, we found that B cells required contact to T cells for up to 60 hours to fully activate naive T cells (Figure 3A). In contrast, T cells became independent of further antigen presentation after 2 to 6 hours of contact to DC (dendritic cells)
1a. How long does it take the T Cell to kill the APC if it is a match?
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4. How can we modify MHC (or other APC surface protein) to release the chemicals normally released by Tregs when a Teff successfully binds MHC?
FoxP3 is transcription factor centrally responsible for Treg function: absence of it leads to IPEX (immunodysregulation polyendocrinopathy enteropathy X-linked syndrome) (a fancy way of saying your body will attack itself on all fronts and life will absolutely suck)
(super interesting imo) we all carry diabetogenic t cells controlled by tregs; malfunction of foxp3 makes us all instant type 1 diabetics
Other New, Relevant Information:
-Engineering the pathway that leads to secretion of the appropriate chemicals by Tregs seems like it could be a pain in the ___. Maybe it would be more feasible to engineer Tregs to express endocytotic behavior/MHC and somehow work it so that their TCRs are stimulated when MHC is bound that way the original exocytotic pathway can be preserved
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- MHC Class I presents peptides from proteins produced by the cell (viral or self) whereas MHC Class II presents endocytosed antigens, If we plan to engineer the production of MHC into a cell that typically does not produce it, we'll probably want both for the most adaptability
- gamma delta t-cells not MHC reliant, recognize whole proteins; human cells recognize non-petidic antigens like HMB-PP, an essential metabolite in pathogenic bacteria that's absent from human host... dope.... maybe has applications for our project??
Just a pretty picture I found ...
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