| Expand | |||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |||||||||||||||||||||||||||||||||
| title | Setup
Well 1 |
|---|
HEK293
Well 2 |
|---|
Well 3 | Well |
|---|
4 |
|---|
HEK293
Well 4Well 5 | Well 6 | ||||
|---|---|---|---|---|---|
untransfected (calibration) | eBFP bleed through control | leaky mKate control | TEVp control (nonspecific G4 cleavage) | TEVp control (nonspecific G4 cleavage) | TEVp control (nonspecific G4 cleavage) |
HEK293 |
| HEK293 |
Well 6
HEK293
Well 7
HEK293
Well 8
HEK293
Well 9
HEK293
Well 10
HEK293
Well 11
HEK293
Well 12
HEK293
Well 13
HEK293
Well 14
HEK293
Well 15
HEK293
Well 16
HEK293
Well 17
HEK293
Well 18
HEK293
Well 19
HEK293
Well 20
HEK293
Well 21
HEK293
Well 22
HEK293
Well 23
HEK293
100 ug hEF1a: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A 100 ug CD79B 100 ug TRE: Syk-TEVp 100 ug Lyn
| HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A 100 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 100 ug Lyn | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A-TCS-G4 100 ug CD79B 100 ug UAS: eYFP
| HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A 100 ug CD79B-TCS-G4 100 ug UAS: eYFP
| 100 ug HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A-TCS-G4 100 ug CD79B-TCS-G4 100 ug UAS: eYFP
| |
| Well 7 | Well 8 | Well 9 | Well 10 | Well 11 | Well 12 |
|---|---|---|---|---|---|
HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A-TCS-G4 100 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox
| HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 50 ug GmabH 50 ug GmabL 50 ug CD79A-TCS-G4 50 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 25 ug GmabH 25 ug GmabL 25 ug CD79A-TCS-G4 25 ug CD79B 100 ug : Syk-TEVp 100 ug UAS: eYFP 10 nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 12.5 ug GmabH 12.5 ug GmabL 12.5 ug CD79A-TCS-G4 12.5 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 6.25 ug GmabH 6.25 ug GmabL 6.25 ug CD79A-TCS-G4 6.25 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 3.125 ug GmabH 3.125 ug GmabL 3.125 ug CD79A-TCS-G4 3.125 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox |
| Well 13 | Well 14 | Well 15 | Well 16 | Well 17 | Well 18 |
HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A 100 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 50 ug GmabH 50 ug GmabL 50 ug CD79A 50 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 25 ug GmabH 25 ug GmabL 25 ug CD79A 25 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 12.5 ug GmabH 12.5 ug GmabL 12.5 ug CD79A 12.5 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 6.25 ug GmabH 6.25 ug GmabL 6.25 ug CD79A 6.25 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox | HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 3.125 ug GmabH 3.125 ug GmabL 3.125 ug CD79A 3.125 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox
|
| Well 19 | Well 20 | Well 21 | Well 22 | Well 23 | Well 24 |
100 ug HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A-TCS-G4 100 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox
| 100 ugHEK293 100 ughEF1a: mKate 100 ughEF1a: rtTA 50 ug GmabH 50 ug GmabL 50 ug CD79A-TCS-G4 50 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox
| 100 ug HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 25 ug GmabH 25 ug GmabL 25 ug CD79A-TCS-G4 25 ug CD79B-TCS-G4 100 ugTRE: Syk-TEVp 100 ug UAS: eYFP 10nM dox
| 100 ug HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 12.5 ug GmabH 12.5 ug GmabL 12.5 ug CD79A-TCS-G4 12.5 ug CD79B-TCS-G4 100 ugTRE: Syk-TEVp 10 nM dox
| 100 ug HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 6.25 ug GmabH 6.25 ug GmabL 6.25 ug CD79A-TCS-G4 6.25 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox
| 100 ug HEK293 100 ug hEF1a: mKate 100 ug hEF1a: rtTA 3.125 ug GmabH 3.125 ug GmabL 3.125 ug CD79A-TCS-G4 3.125 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP 10 nM dox |
Well 24
HEK293| Expand | ||
|---|---|---|
| ||
|
| Expand | ||
|---|---|---|
| ||
|
| Expand | ||
|---|---|---|
| ||
|
| Expand | ||
|---|---|---|
| ||
|
| Expand | ||
|---|---|---|
| ||
|