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THINGS TO DO 

Experiments 

Task

...

PersonDate

Syk-

...

TEVp cleavage with serial dilutions

...

    •  Transfect
    •  Add dox
    •  Cytometry (1.30pm)

CR 

 

 

  •  8/26
  •  8/27
  •  8/28
BCR membrane localization with serial dilution CR 

Cofilin Western Blot

    •  Run gel and trasnfer
    •  Antibody incubations and imaging
LA

 

  •  8/26
  •  8/27

PirB abeta binding live microscopy

    •  Stain and prepare for microscopy (5pm)
LA 

 

  •  8/27

LilrB2 localization cytometry

  •  Transfect
  •  Stain and prepare for cytometry (1.30 pm)
LA

 

  •  8/26
  •  8/28

LilrB2 abeta binding cytometry

  •  Transfect
  •  Stain and prepare for cytometry (1.30 pm)
LA

 

  •  8/26
  •  8/28

TEVp-Cofilin cleavage

  •  Transfect
  •  Prepare for cytometry (1.30 pm)
LA 
  •  8/26
  •  8/28
L7ae dilution seriesGB 
BACE1 and BACE2 localizationJA 
BACE1 and BACE2 assayJA 

 

Data Analysis

Experiment to be AnalyzedPerson
  
  
  
  
  

 

 

Cloning

TaskPersonDate
Lyn-

...

TEVp  

...

BACE1

...

YFP fusion

...

    •  LR
    •  Kill and transform
    •  Pick colonies
    •  Miniprep
    •  Verify pEXPR
    •  Midiprep
  

...

BACE2 YFP fusion

    •  LR
    •  Kill and transform
    •  Pick colonies
    •  Miniprep
    •  Verify pEXPR
    •  Midiprep
  
BCR Gibson  

...

LilrB2-YFP

...

SS 

...

LilrB2-TCS-Gal4VP16SS 

Non-Experimental Stuff 

Task

...

PersonDate
External Wiki  
Survey analysis  
Novartis person (what do we want from her?)