...
- Miniprep LRs
- Digest LRs
- Also digest pEXPR_hEF1a: PirBYFP
- Transform PirB-YFP LRs (maybe do treatment's one too...). Nope.
- PCR LilrB2 for YFP and TCSG4
- Run: Digests, PCRs
- Cut out PCR bands if worked.
- Midi inoculate if LilrB2 LRs worked.
- LR pENTR_PirB:YFP to hEF1a and TREt
...