Todo Today:
- Miniprep LRs
- Digest LRs
- Also digest pEXPR_hEF1a
irBYFP: PirBYFP - Transform PirB-YFP LRs (maybe do treatment's one too...). Nope.
- PCR LilrB2 for YFP and TCSG4
- Run: Digests, PCRs
- Cut out PCR bands if worked.
- Midi inoculate if LilrB2 LRs worked.
- LR pENTR_PirB:YFP to hEF1a and TREt
PCRing at 53 degress, 40 seconds.
...
HL, pEXPR_TREt:LilrB2 (1-4), pEXPR_TREt:LilrB2 C, PCR_LilrB2(Y), PCR_LilrB2(T)
Both LilrB2 pEXPRs worked!