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  •  Miniprep LRs
  •  Digest LRs
  •  Also digest pEXPR_hEF1a(tongue)irBYFP
  •  Transform PirB-YFP LRs (maybe do treatment's one too...)
  •  PCR LilrB2 for YFP and TCSG4
  •  Run: Digests, PCRs
  •  Cut out PCR bands if worked.

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PCRing at 53 degress, 40 seconds.