Page History

To Do

Cloning

•  Sequence A4/B4
  •  A4/B4 1 and 3
•  fix the other Gibsons
•  midi culture TRE:BCR (TRE:CD79A,TRE:CD79B,TRE:GmabL,TRE:GmabH 1,TRE Lyn-mKate)
  
  •  midi prep
•  re digest hef1a:Lyn
•  appSWE?

Gibsons shouldn't exceed 30C, they are big and will recombine strangely at 37

Monday

•  Tissue Culture
  •  Seed 3 plates (2 w/ glass cover slips 1 standard)
  •  Dilute remaining DNA
  •  Update Experiments Pages
    
  •  Experiment Timeline

Friday

•  TC
  •  Dilute Midipreps
  •  Update Experiments Pages
  •  Experiment Timeline
  •  Seed
•  Cloning
  
  •  Plasmids Library
•  Thursday----------------------------------------------------------------------------------------
•  TC
  •  Dilute Midipreps
  •  Stain Slides
  •  Finish Planning
  •  add Dox
  •  Seed?
•  Wednesday-------------------------------------------------------------------------------------
•  TC
  •  Path Foreward
  •  Dilute Midipreps
  •  Transfect
    •  Tango 2
    •  Membrane Localization Dilution Microscopy

Plasmids library

digest APP stuff