Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Re-analyze all of our data in MATLAB (Gary)
- Plan experiments for combinations of the sensorsLearn how to acquire MATLAB and analyze datafrom Brian
- Find natural concentrations of relevant miRNAs.
miRNA Sensors (Lab Work)
- Pull the Transformation plates out of the incubator and check for red and white colonies
- Pick 3-4 Colonies of MAV hEF1a:Tal14 for miniprepping and verification tomorrow
- Mini-prep MAV1212 hEF1A Tal14
- Digest and Map MAV1212 hEF1A Tal14
- Transform third LR trial if not verified
- Pick/Streak hEF1a: Gal4VP16 2A rtTA3