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titlePirB Trial 1

Microscopy

ControlPermeabilizedNon-permeabilizedLilrB2 Paper

Cytometry

Expand
titleGating
SSC-A vs FSC-AFSC-H vs FSC-WSSC-H vs SSC-WYellow vs Red
Tube 1Tube 2Tube 3Tube 4
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titlePirB Trial 3
Negative ControlPirB-YFP only (no transfection marker)
Image AddedImage Added
 

Analysis: 

Cytometry..

LilrB2 and PirB Trial 1: The plots for the mkate only and mkate + receptor cell populations are very similar. This is most probably due to the bleed through of the red channel into the yellow channel (the 'sensitivity' of the PMTs for the yellow channel was increased because the yellow fluorophore was not very bright).

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