Page History

To Do

•      Monday morning
•      Monday afternoon
•          pick colonies from gibsons
•          retransform Lyn, all the TRE parts.
•          Pick colonies from APP, APPSwe
  Gibsons shouldn't exceed 30C, they are big and will recombine strangely at 37

Monday

•  Tissue Culture
  •  Seed 3 plates (2 w/ glass cover slips 1 standard)
  •  Dilute remaining DNA
  •  Update Experiments Pages
    
  •  Experiment Timeline
•  Cloning
  •  Transform
    •  TRE: Lyn-mKate verified mini
      
    •  TRE: Lyn-TEV LR
      
    •  TRE: Gmab L verified mini
      
    •  TRE: CD79B verified mini
      
    •  TRE: CD79A verified mini
      
    •  hEF1a: Lyn LR
      
    •  TRE: Syk-eYFP LR
      
  •  Pick Colonies
    
    •  A/B
    •  A4/B
    •  A/B4
    •  A4/B4
  •  LR
    •  TRE: Gmab H

Friday

•  TC
  •  Dilute Midipreps
  •  Update Experiments Pages
  •  Experiment Timeline
  •  Seed
•  Cloning
  
  •  Plasmids Library
•  Thursday----------------------------------------------------------------------------------------
•  TC
  •  Dilute Midipreps
  •  Stain Slides
  •  Finish Planning
  •  add Dox
  •  Seed?
•  Wednesday-------------------------------------------------------------------------------------
•  TC
  •  Path Foreward
  •  Dilute Midipreps
  •  Transfect
    •  Tango 2
    •  Membrane Localization Dilution Microscopy

Future

Process gibsons and APP stuff: minis, verify, grow midi, midi

grow minis, run minis, verify, grow midi, midi

pEXPR 6?

Plasmids library

digest app stuff