To Do - Monday morning
- Monday afternoon
- pick colonies from gibsons
- retransform Lyn, all the TRE parts.
- Pick colonies from APP, APPSwe
- Gibsons shouldn't exceed 30C, they are big and will recombine strangely at 37
sat:
midiprep midi cultures retransform Lyn and the gibsons (gibson cultures ought not exceed 30C)Monday - Tissue Culture
- Seed 3 plates (2 w/ glass cover slips 1 standard)
- Dilute remaining DNA
- Update Experiments Pages
- Experiment Timeline
- Cloning
- Transform
- TRE: Lyn-mKate
- TRE: Lyn-TEV
- TRE: Gmab L
- TRE: CD79B
- TRE: CD79A
- Pick Colonies
- LR
- TRE: Gmab H
- hEF1a: Lyn
- TRE: Syk-eYFP
Friday - TC
- Dilute Midipreps
- Update Experiments Pages
- Experiment Timeline
- Seed
- Cloning MIDI culture
- GmabL4, CD79A 1-4, CD79B 1-4
- Lyn-mKate, apparently we don't have this
- mini culture
- BCR tre transformations
- GmabH
- Move Lab
- Plasmids Library
- Thursday----------------------------------------------------------------------------------------
- TC
- Dilute Midipreps
- Stain Slides
- Finish Planning
- add Dox
- Seed?
Cloning- Digest/sequence
- A/B
- A/B4
- A4/B
- A4/B4
- TRE: Gmab L
- TRE: CD79A
- TRE: CD79B
- APP
- APPswe
- pEXPR6
- Miniprep
- Transform
- LR?
- TRE: Gmab H Wednesday-------------------------------------------------------------------------------------
- TC
- Path Foreward
- Dilute Midipreps
- Transfect
- Tango 2
- Membrane Localization Dilution Microscopy
- Cloning
- Miniprep
- A/B
- A4/B
- A/B4
- A4/B4
- TRE: Gmab L
- TRE: CD79A
- TRE: CD79B
- APP
- APPswe
- Digest/sequence
- A/B
- A/B4
- A4/B
- A4/B4
- TRE: Gmab L
- TRE: CD79A
- TRE: CD79B
- APP
- APPswe
- Pick Colonies
| Future Process gibsons and APP stuff: minis, verify, grow midi, midi grow minis, run minis, verify, grow midi, midi pEXPR 6? Plasmids library |
|---|