Page History

To Do

•      Monday morning
•          retransform pEXPR tre:GmabH and pEXPR Lyn
•          retransform gibsons and run all incubations at 30C
•      Monday afternoon
•          Inoculate new midis from midis that ran over weekend.

sat:
midiprep midi cultures

retransform Lyn and the gibsons (gibson cultures ought not exceed 30C)

Monday

•  Tissue Culture
  •  Seed 3 plates (2 w/ glass cover slips 1 standard)
  •  Dilute remaining DNA
  •  Update Experiments Pages
    
  •  Experiment Timeline
•  Cloning
  •  Start MidisTransform
    •  TRE: Lyn-mKate
    •  TRE: Lyn-TEV
    •  TRE: Gmab L
    •  TRE: CD79B
    •  TRE: CD79A
      
  •  Transform Gibsons (incubate at 30 degrees) Pick Colonies
    
    •  A/B
    •  A4/B
    •  A/B4
    •  A4/B4
  •  LR
    •  TRE: Gmab H
    •  hEF1a: Lyn
    •  TRE: Syk-eYFP

Friday

•  TC
  •  Dilute Midipreps
  •  Update Experiments Pages
  •  Experiment Timeline
  •  Seed
•  Cloning
  •  MIDI culture
    
    •   GmabL4, CD79A 1-4, CD79B 1-4
    •  Lyn-mKate, apparently we don't have this
      
  •  mini culture
    •  BCR tre transformations
    •  GmabH
      
     
  •  Move Lab
  •  Plasmids Library
•  Thursday----------------------------------------------------------------------------------------
•  TC
  •  Dilute Midipreps
  •  Stain Slides
  •  Finish Planning
  •  add Dox
  •  Seed?
•  Cloning
  •  Digest/sequence
    •  A/B
    •  A/B4
    •  A4/B
    •  A4/B4
    •  TRE: Gmab L
    •  TRE: CD79A
    •  TRE: CD79B
    •  APP
    •  APPswe
    •  pEXPR6
  •  Miniprep
    •  pEXPR6
  •  Transform
    
    •  TRE: Gmab H
    •  hEF1a: Lyn
  •  LR?
    •  TRE: Gmab H
•  Wednesday-------------------------------------------------------------------------------------
•  TC
  •  Path Foreward
  •  Dilute Midipreps
  •  Transfect
    •  Tango 2
    •  Membrane Localization Dilution Microscopy
•  Cloning
  •  Miniprep
    •  A/B
    •  A4/B
    •  A/B4
    •  A4/B4
    •  TRE: Gmab L
    •  TRE: CD79A
    •  TRE: CD79B
    •  APP
    •  APPswe
  •  Digest/sequence
    •  A/B
    •  A/B4
    •  A4/B
    •  A4/B4
    •  TRE: Gmab L
    •  TRE: CD79A
    •  TRE: CD79B
    •  APP
    •  APPswe
  •  Pick Colonies
    
    •  TRE: Gmab H
    •  pEXPR 6

•      Tuesday morning
•          midiprep midicultures
•          pick colonies from gibsons, mini at 30C
•      Tuesday afternoon
•          pick colonies from pEXPR tre:GmabH and pEXPR Lyn
•      Wednesday morning
•          run minis from gibsons, GmabH, and Lyn
•          restriction map gibsons, GmabH, and Lyn
•          send out gibsons, GmabH, and lyn for sequencing
•      Wednesday afternoon
•      Thursday morning
•          Culture midis for gibsons at 30C
•      Thursday afternoon
•          Culture midis from GmabH and Lyn
•      Friday morning
•          Run midis on gisbons and GmabH and Lyn
•      Friday afternoon