Midipreps require a vacuum manifold and minipreps may use it if you so choose. It uses vacuum to draw fluid through a spin column. Our labs manifold was in poor shape, the hose was attached with a quarter inch mass of parafilm and the opposite end from the hose leaked. A new 24 tube vacuum manifold from QIAGEN costs $270, the following kit cost 15.21(even with a lot of leftovers) and holds 36 tubes.
| Proof | ||||
|---|---|---|---|---|
Works like a charm |
| Tools: |
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| something to cut PVC pipe, I used a hacksaw and the wood saw on my multitool. |
| Something to tighten the brass fitting, I used a crescent wrench |
| optional: Hot glue gun or teflon tape to seal brass fitting, probably not needed |
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