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pENTR LilrB2:

  •  Blunt end cloning of gBlock
  •  PCR screen of product

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Image Added

  •  Miniprep sample with correct band pattern
  •  Sequence verify
  •  Proceed with LilrB2 constructs

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pEXPR hEF1a:PirB-YFP

  •  Transform
  •  Grow up midi culture
  •  Midiprep
  •  Make cell stock

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