Page History

To Do

•  Monday-----------------------------------------------------------------------------------------
•  Cloning
  •  Streak Bacterial Stab
    •  hEF1a: APP
    •  hEF1a: APPswe
  •  Transform
    •  TRE: Gmab H
    •  TRE: Gmab L
    •  TRE: CD79A
    •  TRE: CD79B
  •  Miniprep
    •  Lyn
  •  Digest & Gel
    •  Lyn
    •  Lyn-TEV
    •  Syk-eYFP
  •  Grow Midiprep
    •  Lyn
    •  Lyn-TEV
    •  Syk-eYFP
  •  Sequence
    •  hEF1a: Gmab H Midi
    •  hEF1a: Gmab L Midi
    •  hEF1a: CD79A Midi
    •  hEF1a: CD79B Midi
•  Tissue Culture
  •  Finish Platemaps & Timeline
  •  Prep TEV Cleavage System for cytometry
  •  Neuronal Cultures
  •  Seed?
  •  Data analysis & add data to wiki
•  Thursday---------------------------------------------------------------------------------------
•  Tissue Culture
  •  Cytometry for BCR Loc 3
  •  Transfect aBeta Binding 2
  •  Experimental Timeline
  •  Neuronal Test Bed Research
•  Cloning
  •  Propagate Bacterial Stabs
    •  APP
    •  APPswe
  •  Transform
    •  Syk-eYFP
    •  Lyn-TEV
    •  Lyn-STOP
•  Wednesday------------------------------------------------------------------------------------
•  Transform
  •  Syk-eYFP
  •  Lyn
  •  Lyn-TEV
•  Transfect
  •  BCR Localization Test 3
•  Seed?
•  Primary Neuronal Cultures
•  How to fix B-Cells
•  aBeta Binding Microscopy Prep
•  Tuesday------------------------------------------------------------------------------------------
•  Analyze Syk-eYFP Sequencing Data
•  LR
  •  Syk-eYFP
  •  Lyn
  •  Lyn-TEV
•  Transfect something?
•  Powow
•  Pick new promoters for BCR Components
•  Monday-------------------------------------------------------------------------------------------
•  Tissue Culture
  •  aBeta/streptavidin staining protocol (CR)
  •  Oligomerize aBeta (CR)
  •  Cytometry for aBeta binding (CR)
  •  Microscopy prep for aBeta binding (LA)
  •  Run leftover microscopy for BCR&LilrB2 (LA)
  •  Seed Lyn Recruitment (LA)
•  Analyze BCR Localization Data
•  Sequence Syk-eYFP
•  LR Syk-eYFP
•  Transform Lyn-STOP LR?
•  Transform Lyn-TEV LR?
•  Friday----------------------------------------------------------------------------------------------
•  Miniprep pEXPR (Lyn&Lyn-TEVp)
•  digest and gel pEXPR (Lyn&Lyn-TEVp) and pENTR Syk-eYFP
•  Stain for Cytometry
•  Run cytometry
•  Stain for microscopy
•  Design Lyn and Syk Localization
•  Seed for aBeta Binding/Lyn Localization?
•  aBeta Oligomerization
•  Dilute all Midipreps&Label pENTRs
•  Thursday----------------------------------------------------------------------
•  figure out what to do for lyn-TEVp
•  pick/grow up pEXPR Lyn-TEVp, pEXPR Lyn-stop, pENTR Syk-eYFP
•  Backlog----------------------------------------------------------------------
•  Design Experiments
•  Oligomerization of aBeta
•  Fix Calendar
•  Discuss Neurons