Versions Compared

Old Version 110

changes.mady.by.user Unknown User (krbrink@mit.edu)

Saved on

compared with

New Version 111

changes.mady.by.user Unknown User (clrichar@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Syk-TEVp Cleavage

 

  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  Cover Slips
  •  0.1% Gelatin
  •  Sodium Azide
  •  Fixation Buffer
  •  Parafilm
  •  Triton X-100
  •  Embedding Solution
  •  Biotinylated aBeta
  •  Oligomerized Biotinylated aBeta
  •  Streptavidin-RED

Ongoing

Parts/ReagentsParts/Reagents
  •  hEF1a: Gmab H
  •  hEF1a: Gmab L
  •  hEF1a: CD79A-TCS-Gal4VP16
  •  hEF1a: CD79B-TCS-Gal4VP16
  •  hEF1a: Lyn
  •  TRE: Lyn-TEV
  •  TRE: Syk-TEV
  •  hEF1a: rtTA
  •  UAS: eYFP
  •  Biotinylated aBeta
  •  Anti IgM Green
NumberNameParts/ReagentsStatus
1Membrane Localization of BCR 
Expand
titleParts/Reagents
  •  B Cells
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  Cover Slips
  •  0.1% Gelatin
  •  RPMI1640
  •  Sodium Azide
  •  Fixation Buffer
  •  Parafilm
  •  Triton X-100
  •  Embedding Solution
  •  Anti IgM Green

Ongoing

2Beta Amyloid Binding of Gmab in BCR Complex 
Expand
titleParts/Reagents
3Syk Western Blot
Expand
titleParts/Reagents
  •  ?

Complete

4Membrane Localization of Lyn 

Waiting on Dependencies

Expand
titleParts/Reagents
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  TRE: Lyn-mKate
  •  hEF1a: rtTA
  •  Dox
  •  Biotinylated aBeta
5Membrane Localization of Syk
Expand
title hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  hEF1a: Lyn
  •  TRE: Syk-eYFP
  •  hEF1a: rtTA
  •  Biotinylated aBeta
6Inactive TANGO Test
Expand
title
  •  hEF1a: Gmab H
  •  hEF1a: Gmab L
  •  hEF1a: CD79A-TCS-Gal4VP16
  •  hEF1a: CD79B-TCS-Gal4VP16
  •  hEF1a: Lyn
  •  TRE: Lyn-TEV
 TRE: Syk-TEV
  •  hEF1a: rtTA
  •  UAS: eYFP
  •  Dox
Cleavage

 

7Lyn-TEV Cleavage

 

8Syk-TEV & Lyn Cleavage 7Active TANGO Test
Expand
titleParts/Reagents