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| 1 Tre:BACE2+FRET Substrate 2 | 2 Tre:miRG1,Hef1a:BACE1 (0 Dox) +FRET Substrate 1 | 3 Tre:miRG1,Hef1a:BACE1 (1000 Dox) +FRET Substrate 1 | 4 Assay Buffer 2 + FRET Substrate 2 | 5Assay Buffer 1 + FRET Substrate 1 | 6 HEK cells (no substrate) | 7 Purified BACE1 + FRET Substrate 1 | 8 Purified BACE1, BACE1 Inhibitor + FRET Substrate 1* | 9 Tre:miRG1,Hef1a:BACE1 (0 Dox), BACE1 Inhibitor +FRET Substrate 1* | 10 | 11 | 12 |
| 13 5 uM Reference Standard+ FRET 1 | 14 2.5 uM Reference Standard+ FRET 1 | 15 1.25 uM Reference Standard+ FRET 1 | 16 0.63 uM Reference Standard+ FRET 1 | 17 0.32 uM Reference Standard+ FRET 1 | 18 0.16 uM Reference Standard+ FRET 1 | ||||||
| 19 5 uM Reference Standard+ FRET 2 | 20 2.5 uM Reference Standard+ FRET 2 | 21 1.25 uM Reference Standard+ FRET 2 | 22 0.63 uM Reference Standard+ FRET 2 | 23 0.32 uM Reference Standard+ FRET 2 | 24 0.16 uM Reference Standard+ FRET 2 |
Make sure all components are at same temperature
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