Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Meet with Kyle about quantifying repression
miRNA Sensors (Lab Work)
- MiniprepFigure out MATLAB quantification
- Analyze Digest
- pENTR TAL14
- Analyze Sequencing Results
- Double-Input Low Sensor
- Find
- pEXPR hEF1A:TAL14
- pENTR TAL21
- pEXPR hEF1A:TAL21
- LR
- MAV1212-hEF1a-TAL14
- MAV1212-hEF1a-TAL21
- Sequence
- Double-input low sensor
- Digest & run gel
- pENTR: TAL14
- Transfect
- L7ae quantification experiment
miRNA Sensors (Lab Work)
- Add DOX
- L7ae quantification experiment
- LR
- hEF1a-TAL14 (if digest works)
- hEF1a-TAL21 (find entry vector / plate)
NOTES