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Microscopy
Cytometry
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TRIAL 2
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| title | Trial 2 |
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| title | Cytometry Plate |
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Well 1
B Cells
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Well 2
HEK 293
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Well 3
HEK293
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Well 4
HEK293
+ Dummy (500ng)
+ hEF1a:mKate (500ng)
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Well 5
HEK293
+ hEF1a:mKate (500ng)
+ hEF1a:PirB (500ng)
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Well 6
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Well 7
B Cells
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Well 8
HEK 293
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Well 9
HEK293
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Well 10
HEK293
+ Dummy (500ng)
+ hEF1a:mKate (500ng)
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Well 11
HEK293
+ hEF1a:mKate (500ng)
+ hEF1a:PirB (500ng)
Well 12
Analysis:
Cytometry..
LilrB2 and PirB Trial 1: The plots for the mkate only and mkate + receptor cell populations are very similar. This is most probably due to the bleed through of the red channel into the yellow channel (the 'sensitivity' of the PMTs for the yellow channel was increased because the yellow fluorophore was not very bright).
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