...
| Expand |
|---|
|
| Expand |
|---|
| Well 1B Cells | Well 2HEK 293 Permeabilized (Unstained) | Well 3 HEK293 Permeabilized | Well 4 HEK293 + Dummy (500ng) + hEF1a:mKate (500ng) Permeabilized | Well 5HEK293 + hEF1a:mKate (500ng) + hEF1a:PirB (500ng) Permeabilized | Well 6 | Well 7B Cells | Well 8HEK 293 Permeabilized (Unstained) | Well 9 HEK293 Permeabilized | Well 10 HEK293 + Dummy (500ng) + hEF1a:mKate (500ng) Permeabilized | Well 11HEK293 + hEF1a:mKate (500ng) + hEF1a:PirB (500ng) Permeabilized | Well 12 | Well 13B Cells | Well 14HEK 293 Non-Permeabilized (Unstained) | Well 15 HEK293 Non-Permeabilized | Well 16 HEK293 + Dummy (500ng) + hEF1a:mKate (500ng) Non- Permeabilized | Well 17HEK293 + hEF1a:mKate (500ng) + hEF1a:PirB (500ng) Non-Permeabilized | Well 18 | Well 19B Cells | Well 20HEK 293 Non-Permeabilized (Unstained) | Well 21 HEK293 Non-Permeabilized | Well 22 HEK293 + Dummy (500ng) + hEF1a:mKate (500ng) Non- Permeabilized | Well 23HEK293 + hEF1a:mKate (500ng) + hEF1a:PirB (500ng) Non-Permeabilized | Well 24 |
|
| | Expand |
|---|
| Well 1B Cells | Well 2HEK 293 | Well 3 HEK293 | Well 4 HEK293 + Dummy (500ng) + hEF1a:mKate (500ng) | Well 5HEK293 + hEF1a:mKate (500ng) + hEF1a:PirB (500ng) | Well 6 | Well 7B Cells | Well 8HEK 293 | Well 9 HEK293 | Well 10 HEK293 + Dummy (500ng) + hEF1a:mKate (500ng) | Well 11HEK293 + hEF1a:mKate (500ng) + hEF1a:PirB (500ng) | Well 12 |
|
|
|
Analysis:
Cytometry..
LilrB2 and PirB Trial 1: The plots for the mkate only and mkate + receptor cell populations are very similar. This is most probably due to the bleed through of the red channel into the yellow channel (the 'sensitivity' of the PMTs for the yellow channel was increased because the yellow fluorophore was not very bright).
...