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title	Trial 2

Expand

title	Microscopy Plate

Well 1

B Cells

Well 2

HEK 293

Permeabilized

(Unstained)

Well 3

HEK293

Permeabilized

Well 4

HEK293

+ Dummy (500ng)

(500ng)

Permeabilized

Well 5

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:PirB (500ng)

Permeabilized

Well 6

Well 7

B Cells

Well 8

HEK 293

Permeabilized

(Unstained)

Well 9

HEK293

Permeabilized

Well 10

HEK293

+ Dummy (500ng)

(500ng)

Permeabilized

Well 11

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:PirB (500ng)

Permeabilized

Well 12

Well 13

B Cells

Well 14

HEK 293

Non-Permeabilized

(Unstained)

Well 15

HEK293

Non-Permeabilized

Well 16

HEK293

+ Dummy (500ng)

(500ng)

Non- Permeabilized

Well 17

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:PirB (500ng)

Non-Permeabilized

Well 18

Well 19

B Cells

Well 20

HEK 293

Non-Permeabilized

(Unstained)

Well 21

HEK293

Non-Permeabilized

Well 22

HEK293

+ Dummy (500ng)

(500ng)

Non- Permeabilized

Well 23

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:PirB (500ng)

Non-Permeabilized

Well 24

Expand

title	Cytometry Plate

Well 1

B Cells

Well 2

HEK 293

Well 3

HEK293

Well 4

HEK293

+ Dummy (500ng)

+ hEF1a:mKate (500ng)

Well 5

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:PirB (500ng)

Well 6

Well 7

B Cells

Well 8

HEK 293

Well 9

HEK293

Well 10

HEK293

+ Dummy (500ng)

+ hEF1a:mKate (500ng)

Well 11

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:PirB (500ng)

Well 12

Analysis:

Cytometry..