Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Expand
titleTrial 1 Transfection Plan
Expand
titleMicroscopy Plate
Well 1

B Cells

Well 2

HEK 293

 

 

 

Permeabilized

(Unstained)

Well 3

 HEK293


 

 

Permeabilized

Well 4

 HEK293

+ Dummy (500ng)

+ hEF1a:mKate

(500ng)

Permeabilized

Well 5

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:LilrB2 (500ng)

Permeabilized

Well 6
Well 7

B Cells

Well 8

HEK 293

 

 

 

Permeabilized

(Unstained)

Well 9

 HEK293

 

 

 

Permeabilized

Well 10

 HEK293

+ Dummy (500ng)

+ hEF1a:mKate

(500ng)

Permeabilized

Well 11

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:LilrB2 (500ng)

Permeabilized

Well 12

 

Well 13

B Cells

Well 14

HEK 293

 

 

 

Non-Permeabilized

(Unstained)

Well 15

 HEK293

 

 

 

Non-Permeabilized

Well 16

 HEK293

+ Dummy (500ng)

+ hEF1a:mKate

(500ng)

Non-Permeabilized

Well 17

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:LilrB2 (500ng)

Non-Permeabilized

Well 18
Well 19

B Cells

Well 20

HEK 293

 

 

 

Non-Permeabilized

(Unstained)

Well 21

 HEK293

 

 

 

Non-Permeabilized

Well 22

 HEK293

+ Dummy (500ng)

+ hEF1a:mKate

(500ng)

Non-Permeabilized

Well 23

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:LilrB2 (500ng)

Non-Permeabilized

Well 24
Expand
titleCytometry Plate
Well 1

B Cells

Well 2

HEK 293

 

 

 

Well 3

 HEK293

 

Well 4

 HEK293

+ Dummy (500ng)

+ hEF1a:mKate (500ng)

Well 5

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:LilrB2 (500ng)

Well 6
Well 7

B Cells

Well 8

HEK 293

 

 

Well 9

 HEK293

 

 

Well 10

 HEK293

+ Dummy (500ng)

+ hEF1a:mKate (500ng)

Well 11

HEK293

+ hEF1a:mKate (500ng)

+ hEF1a:LilrB2 (500ng)

Well 12

 

 


Results:

Expand
titleLilrB2 Trial 1

Microscopy

PermeabilizedNon-permeabilizedNon-permeabilized stained control 
 


Cytometry

Tube 2Tube 3Tube 4Tube 5
  
Tube 8Tube 9Tube 10Tube 11

Overlay of 4 and 5:

hef1a:mKate + dummy hef1a:mKate + hef1a:LilrB2  
+=

 

 

...