Page History

•  Miniprep pEXPR (hEF1a: PirBTCS, hEF1a: TEVpCofilin)
•  Verify hEF1a: PirBTCS
•  Miniprep pENTR LilrB2TCS
  
•  Verify pENTR LilrB2TCS
•  Midiprep inoculate hEF1a: TCSPirBTCS
•  Pick more TREt:TEVpCofilin
•  Plate more TREt:TEVpCofilin if possible.  Transform more TREt:TEVpCofilin LR
  
  

Digest pEXPR_hEF1a: PirBTCSG4VP16 with BsrGI, buffer 2.1
Digest pENTR_LilrB2:TCSG4VP16 with XhoI +XbaI, buffer CS

Order: hEF1a PT 1, 2, 3, 4, LT16, 17, 18, 19

Image Added

hEF1a: PT 1, 3 and 4 might have worked, given that I can't even see the 800bp of the ladder either. Needs further testing.
LT 16 might have worked: there is a very faint band just below the 1500 place. Should test further, but should also keep searching.

To Do:

Short

•  Take out TC plate and liquid cultures
•  Take out LT liquid cultures
•  Miniprep ^

...

Long

•  Find good TREt:TC <-- super important. Needs to get done by end of this week.
  
•  Find good LT
•  Do hEF1a:LT LR
•  PCR C term YFP
•  
  

...