Page History

To Do

•  Monday-------------------------------------------------------------------------------------------
•  Tissue Culture
  •  aBeta/streptavidin staining protocol (CR)
  •  Oligomerize aBeta (CR)
  •  Cytometry for aBeta binding (CR)
  •  Microscopy prep for aBeta binding (LA)
  •  Run leftover microscopy for BCR&LilrB2 (LA)
  •  Seed Lyn Recruitment (LA)
•  Analyze BCR Localization Data
•  Sequence Syk-eYFP
•  LR Syk-eYFP
•  Transform Lyn-STOP LR?
•  Transform Lyn-TEV LR?
•  Friday----------------------------------------------------------------------------------------------
•  Miniprep pEXPR (Lyn&Lyn-TEVp)
•  digest and gel pEXPR (Lyn&Lyn-TEVp) and pENTR Syk-eYFP
•  Stain for Cytometry
•  Run cytometry
•  Stain for microscopy
•  Design Lyn and Syk Localization
•  Seed for aBeta Binding/Lyn Localization?
•  aBeta Oligomerization
•  Dilute all Midipreps&Label pENTRs
•  Thursday----------------------------------------------------------------------
•  figure out what to do for lyn-TEVp
•  pick/grow up pEXPR Lyn-TEVp, pEXPR Lyn-stop, pENTR Syk-eYFP
•  Backlog----------------------------------------------------------------------
•  Design Experiments
•  Oligomerization of aBeta
•  Fix Calendar
•  Discuss Neurons