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  •  Miniprep pEXPRs (TREt TC, hEF1a YC)
  •  Check pEXPRs (TREt TC, hEF1a YC)
  •  Inoculate Midipreps
  •  Pick colonies for hEF1a LT, PT, TC

Digest TREtTC with ApaI Cutsmart
Digest hEF1aYC with BsrGI 2.1
Digest LT with BglI 3.1