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titleSetup

Well 1

HEK293

 

Well 2

HEK293

eBFP

Dummy DNA

Well 3

HEK293

eBFP

Lyn-mKate

Dummy DNA

Well 43

HEK293eBFP

GmabH

GmabL

CD79A

CD79B

Lyn-mKate

Well 4

EMPTY

Well 5 

EMPTY

 

Well 6 

EMPTY

 

Well 7

HEK293

Oligomerized Beta Amyloid

Well 8 

HEK293eBFP

Lyn-mKate

Dummy DNA

Oligomerized Beta Amyloid

Well 9 

HEK293

GmabH

GmabL

CD79A

CD79BeBFP

Lyn-mKate

Dummy DNA

Oligomerized Beta Amyloid (conc. 1)

Well 10 

HEK293eBFP

GmabH

GmabL

CD79A

CD79B

Lyn-mKate

Oligomerized Beta Amyloid (conc. 2)

Well 11 

EMPTY

 

Well 12 

EMPTY

 

Well 13

HEK293

Monomerized Beta Amyloid

Well 14

HEK293eYFP

Lyn-mKate

Dummy DNA

Monomerized Beta Amyloid

Well 15

HEK293

GmabH

GmabL

CD79A

CD79BeYFP

Lyn-mKateDummy DNA

Monomerized Beta Amyloid (conc. 1)

Well 16

HEK293

eYFP

GmabH

GmabL

CD79A

CD79B

Lyn-mKate

Monomerized Beta Amyloid (conc. 2)

Well 17

EMPTY


Well 18

EMPTY


Well 19

HEK293

Anti IgM

Well 20

HEK293eYFP

Lyn-mKate

Dummy DNA

Anti IgM

Well 21

HEK293

GmabH

GmabL

CD79A

CD79BeYFP

Lyn-mKate

Dummy DNA

Anti IgM (conc. 1)

Well 22

HEK293eYFP

GmabH

GmabL

CD79A

CD79B

Lyn-mKate

Anti IgM (conc. 2)

Well 23

EMPTY


Well 24

EMPTY


Expand
titleExpected Results

Lyn should localize in at least some samples. Hopefully Lyn will only localize if the BCR is active and will not localize if it is inactive.

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