...
- Miniprep PY, LY, pENTR_TEVpCofilin
- Verify Minipreps
- Transform hEF1a:YFPCofilin
- Pick TREt:YFPCofilin Colonies
- LR pENTR_TEVpCofilin with hEf1a and TREt
- LR LT 12 and PT 11 with hEf1a
...
All 4 pENTR_TEVpCofilin worked!
...
Of course, the others didn't work. Given the lack of blue colonies, I'm thinking backbone religation/only one part present (probably YFP). Need to figure out something else.
LilrB2TCSG4 seems to have worked! 11 and 13 might have point mutations (high G content, unlikely). Will proceed with 12.
PirBTCSG4 seems to have worked, too! 13 seems to have a point mutation (high G content, unlikely). Will proceed with 11.