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NumberNameParts/Reagents
1Membrane Localization of BCR 
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titleParts/Reagents
  •  B Cells
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  Cover Slips
  •  0.1% Gelatin
  •  RPMI1640
  •  Sodium Azide
  •  Fixation Buffer
  •  Parafilm
  •  Triton X-100
  •  Embedding Solution
  •  Anti IgM Green
2Beta Amyloid Binding of Gmab in BCR Complex 
Expand
titleParts/Reagents
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  Cover Slips
  •  0.1% Gelatin
  •  Sodium Azide
  •  Fixation Buffer
  •  Parafilm
  •  Triton X-100
  •  Embedding Solution
  •  Biotinylated aBeta
  •  Oligomerized Biotinylated aBeta
  •  Streptavidin-RED
3Syk Western Blot
Expand
titleParts/Reagents
  •  ?
4Membrane Localization of Lyn 
Expand
titleParts/Reagents
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  TRE: Lyn-mKate
  •  hEF1a: rtTA
  •  Dox
  •  Biotinylated aBeta
5Membrane Localization of Syk
Expand
titleParts/Reagents
  •  hEF1a: Gmab L
  •  hEF1a: Gmab H
  •  hEF1a: CD79A
  •  hEF1a: CD79B
  •  hEF1a: Lyn
  •  TRE: Syk-eYFP
  •  hEF1a: rtTA
  •  Biotinylated aBeta
6Inactive TANGO Test
Expand
titleParts/Reagents
  •  hEF1a: Gmab H
  •  hEF1a: Gmab L
  •  hEF1a: CD79A-TCS-Gal4VP16
  •  hEF1a: CD79B-TCS-Gal4VP16
  •  hEF1a: Lyn
  •  TRE: Lyn-TEV
  •  TRE: Syk-TEV
  •  hEF1a: rtTA
  •  UAS: eYFP
  •  Dox
7Active TANGO Test
Expand
titleParts/Reagents
  •  hEF1a: Gmab H
  •  hEF1a: Gmab L
  •  hEF1a: CD79A-TCS-Gal4VP16
  •  hEF1a: CD79B-TCS-Gal4VP16
  •  hEF1a: Lyn
  •  TRE: Lyn-TEV
  •  TRE: Syk-TEV
  •  hEF1a: rtTA
  •  UAS: eYFP
  •  Biotinylated aBeta
  •  Anti IgM Green