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titleParts Needed
Parts
pEXPR hEF1a: Gmab Heavy
pEXPR hEF1a: Gmab Light
pEXPR hEF1A: CD79A+stop
pEXPR hEF1a: CD79B+stop
pEXPR hEF1a: eBFP
anti-IgM
beta amyloid
streptavidin-fluorophore (Red)
Expand
titleSetup

 

 

Well 1

Ramos Cells

 HEK293

Well 2

Ramos Cells

1000 nM aBeta

HEK293

Streptavidin

Well 3

HEK293

1000nM

Streptavidin

Well 4

HEK293

1000 nM aBeta

Streptavidin

 

Well 5

HEK293

Dummy

eYFP

1000 nM aBeta

 

Well 6

HEK293

Gmab L

Gmab H

CD79A

CD79B

eYFP

1000 nM aBeta

 

Well 7

Ramos Cells 

Well 8

HEK293

 

Well 9

HEK293 

Dummy

eYFP

Well 10

HEK293

Gmab L

Gmab H

CD79A

CD79B

eYFP

 

Well 11

EMPTY 

Well 12

EMPTY 

Well 13

Ramos Cells

NO COVER SLIP! 

Well 14

Ramos Cells

NO COVER SLIP!

 

Well 15

Ramos Cells

NO COVER SLIP!

 

Well 16

Ramos Cells

NO COVER SLIP! 

Well 17

EMPTY

 

Well 18EMPTY

 

Well 19

EMPTY

Well 20

EMPTY

Well 21

EMPTY

Well 22

EMPTY

Well 23

EMPTY

Well 24

EMPTY

 

 

 

Well 1

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0 nM abeta oligomer

Well 2

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0.1 nM abeta oligomer

Well 3

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0.25 nM abeta oligomer

Well 4

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0.5 nM abeta oligomer

Well 5

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

1 nM abeta oligomer

Well 6

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

2.5 nM abeta oligomer

Well 7

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

5 nM abeta oligomer

Well 8

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

10 nM abeta oligomer

Well 9

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

25 nM abeta oligomer

Well 10

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

50 nM abeta oligomer

Well 11

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

100 nM abeta oligomer

Well 12

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

1000 nM abeta oligomer

Well 13

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0 nM abeta oligomer

Well 14

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

.1 nM abeta

Well 15

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

.25 nM abeta

Well 16

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

.5 nM abeta

Well 17

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

1 nM abeta

Well 18

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

2.5 nM abeta

Well 19

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

5 nM abeta

Well 20

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

10 nM abeta

Well 21

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

25 nM abeta

Well 22

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

50 nM abeta

Well 23

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

100 nM abeta

Well 24

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

1000 nM abeta

...